Temperature-Sensitive Mutants of Influenza A Virus. XIV. Production and Evaluation of Influenza A/Georgia/74-ts-I[E] Recombinant Viruses in Human Adults
Identifieur interne : 002A50 ( Main/Exploration ); précédent : 002A49; suivant : 002A51Temperature-Sensitive Mutants of Influenza A Virus. XIV. Production and Evaluation of Influenza A/Georgia/74-ts-I[E] Recombinant Viruses in Human Adults
Auteurs : Douglas D. Richman [États-Unis] ; Brian R. Murphy [États-Unis] ; Robert B. Belshe [États-Unis] ; Harold M. Rusten [États-Unis] ; Robert M. Chanock [États-Unis] ; Neil R. Blacklow [États-Unis] ; Thomas A. Parrino [États-Unis] ; Frederick B. Rose [États-Unis] ; Myron M. Levine [États-Unis] ; Ellis Caplan [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1977.
Abstract
The two temperature-sensltlve (ts) lesions present in influenza A/Hong Kong/68-ts- 1[E] (H3N268) virus were transferred via genetic reassortment to influenza A/Georgia/74 (H3N274) wild-type virus. A recombinant clone possessing both ts lesions and the shutoff temperature of 38 C of the Hong Kong/68 ts donor and the two surface antigens of the Georgia/74 wild-type virus was administered to 32 seronegative adult volunteers. Thirty-one volunteers were infected, of whom only five experienced mild afebrile upper respiratory tract illness. The wild-type recipient virus was a cloned population that induced illness in five of six infected volunteers. Therefore, the attenuation exhibited by the Georgia/74-ts-l[E] virus could reasonably be assumed to be due to the acquisition of the two ts-1[E] lesions by the Georgia/74 wild-type virus. The serum and nasal wash antibody responses of the ts-l [E] vaccinees were equivalent to those of the volunteers who received wild-type virus. The two ts lesions present in the Hong Kong/68-ts-1[E] virus have now been transferred three times to a wild-type virus bearing a new hemagglutinin, and in each instance the new ts recombinant exhibited a similar, satisfactory level of attenuation and antigenicity for adults. It seems likely that the transfer of the ts-1[E] lesions to any new influenza virus will regularly result in attenuation of a recombinant virus possessing the new surface antigens.
Url:
DOI: 10.1093/infdis/136.2.256
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000D65
- to stream Istex, to step Curation: 000D65
- to stream Istex, to step Checkpoint: 001628
- to stream Main, to step Merge: 002C11
- to stream Main, to step Curation: 002A50
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Temperature-Sensitive Mutants of Influenza A Virus. XIV. Production and Evaluation of Influenza A/Georgia/74-ts-I[E] Recombinant Viruses in Human Adults</title><author><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name></author><author><name sortKey="Belshe, Robert B" sort="Belshe, Robert B" uniqKey="Belshe R" first="Robert B." last="Belshe">Robert B. Belshe</name></author><author><name sortKey="Rusten, Harold M" sort="Rusten, Harold M" uniqKey="Rusten H" first="Harold M." last="Rusten">Harold M. Rusten</name></author><author><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M." last="Chanock">Robert M. Chanock</name></author><author><name sortKey="Blacklow, Neil R" sort="Blacklow, Neil R" uniqKey="Blacklow N" first="Neil R." last="Blacklow">Neil R. Blacklow</name></author><author><name sortKey="Parrino, Thomas A" sort="Parrino, Thomas A" uniqKey="Parrino T" first="Thomas A." last="Parrino">Thomas A. Parrino</name></author><author><name sortKey="Rose, Frederick B" sort="Rose, Frederick B" uniqKey="Rose F" first="Frederick B." last="Rose">Frederick B. Rose</name></author><author><name sortKey="Levine, Myron M" sort="Levine, Myron M" uniqKey="Levine M" first="Myron M." last="Levine">Myron M. Levine</name></author><author><name sortKey="Caplan, Ellis" sort="Caplan, Ellis" uniqKey="Caplan E" first="Ellis" last="Caplan">Ellis Caplan</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:55B3969571E2E6D53CF2B61C1C8DAC704A21654B</idno><date when="1977" year="1977">1977</date><idno type="doi">10.1093/infdis/136.2.256</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-40KN31DJ-Q/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000D65</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D65</idno><idno type="wicri:Area/Istex/Curation">000D65</idno><idno type="wicri:Area/Istex/Checkpoint">001628</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001628</idno><idno type="wicri:doubleKey">0022-1899:1977:Richman D:temperature:sensitive:mutants</idno><idno type="wicri:Area/Main/Merge">002C11</idno><idno type="wicri:Area/Main/Curation">002A50</idno><idno type="wicri:Area/Main/Exploration">002A50</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Temperature-Sensitive Mutants of Influenza A Virus. XIV. Production and Evaluation of Influenza A/Georgia/74-<hi rend="italic">ts</hi>-I[E] Recombinant Viruses in Human Adults</title><author><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation><affiliation></affiliation></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 7, Room 301, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Belshe, Robert B" sort="Belshe, Robert B" uniqKey="Belshe R" first="Robert B." last="Belshe">Robert B. Belshe</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Rusten, Harold M" sort="Rusten, Harold M" uniqKey="Rusten H" first="Harold M." last="Rusten">Harold M. Rusten</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M." last="Chanock">Robert M. Chanock</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Blacklow, Neil R" sort="Blacklow, Neil R" uniqKey="Blacklow N" first="Neil R." last="Blacklow">Neil R. Blacklow</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Parrino, Thomas A" sort="Parrino, Thomas A" uniqKey="Parrino T" first="Thomas A." last="Parrino">Thomas A. Parrino</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Rose, Frederick B" sort="Rose, Frederick B" uniqKey="Rose F" first="Frederick B." last="Rose">Frederick B. Rose</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Levine, Myron M" sort="Levine, Myron M" uniqKey="Levine M" first="Myron M." last="Levine">Myron M. Levine</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Caplan, Ellis" sort="Caplan, Ellis" uniqKey="Caplan E" first="Ellis" last="Caplan">Ellis Caplan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>From the Division of Adult Infectious Diseases, University Hospital, Boston, Massachusetts, Baltimore, Bethesda</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1977-08">1977</date><biblScope unit="vol">136</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="256">256</biblScope><biblScope unit="page" to="262">262</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The two temperature-sensltlve (ts) lesions present in influenza A/Hong Kong/68-ts- 1[E] (H3N268) virus were transferred via genetic reassortment to influenza A/Georgia/74 (H3N274) wild-type virus. A recombinant clone possessing both ts lesions and the shutoff temperature of 38 C of the Hong Kong/68 ts donor and the two surface antigens of the Georgia/74 wild-type virus was administered to 32 seronegative adult volunteers. Thirty-one volunteers were infected, of whom only five experienced mild afebrile upper respiratory tract illness. The wild-type recipient virus was a cloned population that induced illness in five of six infected volunteers. Therefore, the attenuation exhibited by the Georgia/74-ts-l[E] virus could reasonably be assumed to be due to the acquisition of the two ts-1[E] lesions by the Georgia/74 wild-type virus. The serum and nasal wash antibody responses of the ts-l [E] vaccinees were equivalent to those of the volunteers who received wild-type virus. The two ts lesions present in the Hong Kong/68-ts-1[E] virus have now been transferred three times to a wild-type virus bearing a new hemagglutinin, and in each instance the new ts recombinant exhibited a similar, satisfactory level of attenuation and antigenicity for adults. It seems likely that the transfer of the ts-1[E] lesions to any new influenza virus will regularly result in attenuation of a recombinant virus possessing the new surface antigens.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name></region><name sortKey="Belshe, Robert B" sort="Belshe, Robert B" uniqKey="Belshe R" first="Robert B." last="Belshe">Robert B. Belshe</name><name sortKey="Blacklow, Neil R" sort="Blacklow, Neil R" uniqKey="Blacklow N" first="Neil R." last="Blacklow">Neil R. Blacklow</name><name sortKey="Caplan, Ellis" sort="Caplan, Ellis" uniqKey="Caplan E" first="Ellis" last="Caplan">Ellis Caplan</name><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M." last="Chanock">Robert M. Chanock</name><name sortKey="Levine, Myron M" sort="Levine, Myron M" uniqKey="Levine M" first="Myron M." last="Levine">Myron M. Levine</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><name sortKey="Parrino, Thomas A" sort="Parrino, Thomas A" uniqKey="Parrino T" first="Thomas A." last="Parrino">Thomas A. Parrino</name><name sortKey="Rose, Frederick B" sort="Rose, Frederick B" uniqKey="Rose F" first="Frederick B." last="Rose">Frederick B. Rose</name><name sortKey="Rusten, Harold M" sort="Rusten, Harold M" uniqKey="Rusten H" first="Harold M." last="Rusten">Harold M. Rusten</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A50 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002A50 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:55B3969571E2E6D53CF2B61C1C8DAC704A21654B
|texte= Temperature-Sensitive Mutants of Influenza A Virus. XIV. Production and Evaluation of Influenza A/Georgia/74-ts-I[E] Recombinant Viruses in Human Adults
}}
| This area was generated with Dilib version V0.6.33. |  |